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RNA velocity analysis with scVelo. Estimate cell state transitions from unspliced/spliced mRNA dynamics, infer trajectory directions, compute latent time, and…
scVelo — RNA Velocity Analysis Overview scVelo is the leading Python package for RNA velocity analysis in single-cell RNA-seq data. It infers cell state transitions by modeling the kinetics of mRNA splicing — using the ratio of unspliced (pre-mRNA) to spliced (mature mRNA) abundances to determine whether a gene is being upregulated or downregulated in each cell. This allows reconstruction of developmental trajectories and identification of cell fate decisions without requiring time-course data. Installation: pip install scvelo Key resources: Documentation: https://scvelo.readthedocs.io/ GitHub: https://github.com/theislab/scvelo Paper: Bergen et al. (2020) Nature Biotechnology. PMID: 32747759 When to Use This Skill Use scVelo when:
don't have the plugin yet? install it then click "run inline in claude" again.